Metabolomics Unit

Descripción

FUNCTIONS:

• Metabolic profiles using Nuclear Magnetic Resonance (NMR) in tissues, biopsies, bio-fluids and cultures.

• Microscopy imaging using NMR in tissues and small samples (insects, organs of animal models, etc.).

• Molecular imaging in tissues and small samples (insects, organs of animal models, etc.).

• Clinical Magnetic Resonance Imaging using multivariate mathematical models.

Aplicaciones

• Development of new diagnostic tools, prognostic and predictive based on NMR metabolomic profiles for patient stratification in cardiometabolic disease

• Development of new diagnostic tools, prognostic and predictive based on NMR metabolomic profiles for patient stratification in glioblastoma multiforme

• Development of new diagnostic tools, prognostic and predictive based on NMR metabolomic profiles for patient stratification in sepsis

Servicios ofertados

EQUIPMENTS:

• Bruker AVANCE 600 MHz NMR Spectrometer with spectroscopic probes for determination in semi-solid (HR-MAS 1H/13C/15N) and liquid (QXI 1H/13C/15N/31P) systems and micro-imaging probes (10mm/5mm/2mm).

• BCU-Xtreme cooling unit for low-temperature measurements.

• Gilson 215 for automated sample preparation and pipetter, used for the automated preparation of liquid samples in NMR tubes.

• Upright ultra-low temperature freezer (-112ºF) for the storage of samples and for longer terms. Cryogenic container with liquidnitrogen (-320.8 ºF).

Current equipment allows the researchers to work with intact samples (500μ liquid or 10-40 mg tissue) so that, once measured, it is possible to carry out other tests.

Probes available allow researchers to obtain the metabolic profile in liquid samples with a volume of 500μ (5mm tubes) and with a volume of 50μ (1mm capillaries).