This Genomics Unit supports research with the service-based platforms of quality of nucleic acids, sequencing and arrays.
This unit performs research projects on-demand and genetic diagnosis services for hospitals.
- Thermo Scientific NanoDrop 2000c Spectophotometer
- Qubit v2.0 Invitrogen
- Qiagen Qiaxcel (High-Resolution Capillary Electrophoresis)
- 2100 Agilent Bioanalyzer
- GeneChip® Scanner 3000 7G (Affymetrix system)
- ViiA7 Applied Biosystems (Real-Time PCR system)
- ABI Prism 3500 Applied Biosystems Sequencer
- 454 GS Junior Roche Sequencer
- PGM Sequencer and Ion Proton Ion Torrent Sequencer
The Genomics Unit, in collaboration with the Biobank La Fe, enables conducting projects from any clinical sample (blood, tissue or other biological fluids) or from biological collections until the final analysis of results.
SERVICES AND EXPERTISSE
QUALITY OF NUCLEIC ACIDS SERVICE
- Determination of concentration and contaminants of nucleic acids, RNA and DNA by spectrophotometry and fluorimetry (NanoDrop and Qubit).
- Determination of the integrity of nucleic acids (DNA and RNA) by electrophoresis (conventional agarose gels and Bioanalyser microfluidics).
- Genomics, molecular karyotyping and expression arrays ,RNA and microRNA, (Affymetrix Platform)
- Karyotype and molecular genotyping (constitutional and tumour DNA) in a single assay.
- Arrays for genome-wide association studies (GWAS).
- Expression profile of messenger RNA (genes annotated forms of 'splicing' ...)
- Non-coding RNAs expression profile (microRNA, snoRNA ...)
- Analysis of arrays results using PARTEK GENOMIC SUITE and Affymetrix softwares.
- Sanger or conventional sequencing. using the ABI Prism 3500 Applied Biosystems
- Massive sequencing or NGS using 454 GS Junior Roche, PGM and Proton Ion Torrent sequencers.
The platform can carry out a variety of projects:
-De novo resequencing and sequencing of complete genomes, exomes and transcriptomes.
-Design, sequencing and analysis of gene-specific panels of certain pathologies.
-Resequencing of genomic regions of interest for the detection of somatic and germinal variations.
- Conventional PCR
- Quantitative PCR using Applied Biosystems ViiA7