Proteomics

Developed by: Instituto de Investigación Sanitaria Gregorio Marañón

Description

It is a Research Support Service that not only identifies isolated proteins but also allow the massive identification of proteins that make up the proteome. Quantitative proteomics studies can be done to compare the relative abundance of proteins in different samples. In addition, studies of protein interactions with other proteins, and studies of posttranslational modifications such as phosphorylation can be also performed.

Applications

Mass spectrometry

  • Determination of molecular protein weight.
  • Peptide Sequencing

Identification of proteins by peptide fingerprinting (MS)

  • Identification of proteins by peptide fragmentation (ms / ms)

Mass spectrometry coupled to liquid chromatography

  • Analysis of high complexity samples
  • Analysis of subproteomas
  • Surface protein cell study analysis

Differential expression studies

  • DIGE fluorochrome marking
  • ITRAQ isotopic marking
  • Metabolic marking SILAC

Separation of proteins

  • SDS-PAGE
  • 2D-PAGE
  • Isoelectric focusing in liquid phase
  • Blue Native

Separation of peptides

  • Reverse Phase Chromatography
  • ion exchange chromatography
  • Isoelectric focusing in liquid phase

Studies of post-translational modifications. Phosphoproteomic

Isolation of cells by laser capture microdissection (LCM).