Proteomics Unit

Developed by: Instituto de Investigación Sanitaria Fundación Jiménez Díaz

Description

The IIS-FJD features nano/capillary/micro workflows (1200 Series) for integrated proteomic and metabolomic analyses. In particular, the unit is equipped with the instruments necessary to carry out unidimensional and multidimensional liquid chromatography (micro, nano, or capillary) in complex biological samples of peptides, proteins, and/or metabolites. The module-based system has an automatic injector that allows for robotic sample analysis with multidimensional chromatography and online mass spectrometry using a triple quadrupole mass spectrometry (TQMS) with a Chip Cube and JetStream interface. TQMS systems are designed for targeted analysis using selected reaction monitoring (SRM), allowing for quantitative differential analysis of proteins or metabolites of interest. 

Together, the proteomics and genomics units work toward the following aims: 
  • Set up new lines of research in cell therapy, biomarkers, biomaterials, and design of new drugs. 
  • Perform functional analyses and validation of biomarkers for prediction and prognosis. 
  • Further the study of molecular alterations. 
  • Use new drugs for molecular targets.