Multiphoton Microscopy

Developed by: Centro Andaluz de Nanomedicina y Biotecnología BIONAND


Multi-photon (or two-photon) microscopy takes advantages of the near simultaneous absorption of two or more photons which act to excite a fluorescent molecule with the combined energy of both photons.  In practice this means that low energy infrared light can be used to see fluorescent molecules that are normally excited by high energy ultraviolet and blue light.  Using this method we can visualize fluorescent molecules at much greater depths than conventional microscopy.  Infrared light is also much less damaging to live tissues than UV or blue ex-citation, making it ideal for timelapse imaging of model organisms or tissue explants.  The Leica SP5 MP HyD combines both confocal and multi-photon modes in a single machine and can easily switch between different imaging methods.  It is also fitted with the latest super-sensitive external HyD hybrid detectors dedicated for multi-photon microscopy.


Leica SP5 HyD MP Multiphoton Confocal Microscope


  • Deep tissue imaging: imaging
  • Long term live imaging
  • High resolution intravital imaging
  • Quantitative photoactivation
  • Fluorescence properties of novel materials

John Robert Pearson

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