High Content Screening

Developed by: Centro Andaluz de Nanomedicina y Biotecnología BIONAND

Description

High content screening (HCS) attempts to bridge the gap between microscopy and cytometry, providing both large quantities of statically useful and phenotypically rich data.  High content screening combines automated microscopy with customizable algorithms able to detect and quantify many different types of phenotypic information in both fixed and living cultured cells.   The Perkin Elmer Operetta HCS system combines the quality of a spinning disc confocal microscope with the Harmony HCS software, which contains many common algorithms such as nuclei and cells detection, as well as the ability to "learn" new phenotypes.   Bionand also offers access to the advanced server-based Columbus HCS software.


Equipment:

Perkin Elmer Operetta HCS confocal

Applications

  • Drug dose response curve calculation
  • Small molecule screens based on changes in cell phenotype
  • Large scale siRNA screens
  • Long term cell culture studies

Juan Félix López

jflopez@bionand.es

952 367 607