Synaptic Plasticity Unit

Developed by: Instituto de Investigación Sanitaria del Hospital Ramón y Cajal (IRYCIS)

Description

What we offer:

Experience in the characterization of neuronal activity to know the impact of genetic modifications or neuroactive molecules on Central Nervous System functions. We offer three characterizations packages:

1) Characterization of synaptic and neuronal excitability in hippocampal slices.

  • Stimulus-response curves of excitatory synaptic potentials.
  • Synaptic site of actions (presynaptic vs postsynaptic).
  • Resting membrane potential and input resistance.
  • Action potentials: threshold, amplitude and firing rate.
  • Fiber volley (compound action potential of the axons).
  • Monosynaptic inhibitory potentials mediated by GABAA and GABAB receptors.

2) Characterization of synaptic plasticity in hippocampal slices.

It will be evaluated several processes of synaptic plasticity involving glutamate-mediated neurotransmission.In both controls and experimental groups (at least 10 animals per group) it will be performed the following experimental procedures:

  • Stimulus-response curves of excitatory synaptic potentials.
  • Synaptic site of actions (presynaptic vs postsynaptic).
  • Early phase of Long-Term Potentiation (E-LTP). Process with underlying mechanisms independent of protein synthesis.
  • Late phase of Long-Term Potentiation (L-LTP). Process dependent on protein synthesis.
  • Long-term depression (LTD) induced by different stimulation paradigms.
  • Depotentiation (LTP reversal).

3) In vivo brain microdialysis to collect metabolites and neurotransmitters from the extracellular space.

  • Samples will be obtained under basal conditions and stimulated by a secretagogue (high potassium, 4-aminopyridine, etc).

A final report, including figures and interpretations of results, will be provided at the end of study.

Applications

Contact

Scientific Coordinators:

  • José María Solís, PhD.
  • Dra. María Dolores Muñoz, PhD.
Phone numbers: +34 91 336 83 83 /+34 91 336 83 20
e-mail: jose.m.solis@hrc.es  / dolores.munoz@hrc.es