Synaptic Plasticity Unit

What we offer:

Experience in the characterization of neuronal activity to know the impact of genetic modifications or neuroactive molecules on Central Nervous System functions. We offer three characterizations packages:

1) Characterization of synaptic and neuronal excitability in hippocampal slices.

• Stimulus-response curves of excitatory synaptic potentials.
• Synaptic site of actions (presynaptic vs postsynaptic).
• Resting membrane potential and input resistance.
• Action potentials: threshold, amplitude and firing rate.
• Fiber volley (compound action potential of the axons).
• Monosynaptic inhibitory potentials mediated by GABA_A and GABA_B receptors.

2) Characterization of synaptic plasticity in hippocampal slices.

It will be evaluated several processes of synaptic plasticity involving glutamate-mediated neurotransmission.In both controls and experimental groups (at least 10 animals per group) it will be performed the following experimental procedures:

• Stimulus-response curves of excitatory synaptic potentials.
• Synaptic site of actions (presynaptic vs postsynaptic).
• Early phase of Long-Term Potentiation (E-LTP). Process with underlying mechanisms independent of protein synthesis.
• Late phase of Long-Term Potentiation (L-LTP). Process dependent on protein synthesis.
• Long-term depression (LTD) induced by different stimulation paradigms.
• Depotentiation (LTP reversal).

3) In vivo brain microdialysis to collect metabolites and neurotransmitters from the extracellular space.

• Samples will be obtained under basal conditions and stimulated by a secretagogue (high potassium, 4-aminopyridine, etc).

A final report, including figures and interpretations of results, will be provided at the end of study.