Molecular Epidemiology of HIV-1

Developed by: Instituto de Investigación Sanitaria del Hospital Ramón y Cajal (IRYCIS)



The Laboratory of Molecular Epidemiology of HIV-1, led by Dr. Africa Holguin, participates in research projects at national and international levels with public and private funding; both in adults and pediatric HIV infected Spain and other countries. The group collaborates with national AIDS Network (Cohort CoRIS), with HIV Biobank, with the National Center for Tropical Medicine, with a pediatric cohort of children and adolescents infected with HIV in the Community of Madrid, with the Sandoval Center of Health, and with the Department of Infectious and Tropical Medicine of Ramón y Cajal Hospital in Madrid. The group is also integrated into the 33 group for Biomedical Network Research in Epidemiology and Public Health (CIBERESP). Moreover, collaborates with international research groups and participates in international cooperation projects in the field of HIV supported by the National AIDS Plan under the Esther Project, supported by the Spanish Agency for Cooperation and Development (AECID), the Fund Global, working directly with ministries of health from other countries.

Equipment / Know-How of the relevant group

The Laboratory of Molecular Epidemiology of HIV-1 was completely renovated in December 2010. It was designed following the guidelines of WHO for similar laboratories and to ensure the highest quality of processes to guarantee excellence of the work done in the laboratory.
The main areas of work of the group are the genetic variability of HIV and characterization of variants, the identification of resistance to antiretroviral drugs and the study of epidemiological impact, diagnostic and treatments of infections with non-B subtypes and recombinants of HIV 1. Another area of interest is the optimization of methods for monitoring HIV infection cheaper and simpler for implementation in low-income countries.
The Laboratory of Molecular Epidemiology is in the process of accreditation as a specialist HIV reference laboratory of the World Health Organization (WHO) for the detection of resistance in HIV using different types of samples (including dried blood on filter paper: Dried DBS or blood spots) within the WHO Network RESNET.


All the services offered by the laboratory of Molecular Epidemiology of HIV-1 include:
• Very competitive prices and high quality procedures.
• Personalized service and online counseling for queries or resolve doubts by certified personnel.
• Experiments performed in a specialized laboratory with extensive experience and proven scientific rigor and accreditation process by WHO. Individual treatment of each sample.
• Detection and interpretation of genetic resistance to antiretroviral drugs in HIV-1 strains of any subtype or recombinant samples from:
....-whole blood
....- dried blood collected on filter paper (DBS)
....- Plasma or serum
....- infected cells or tissues
• Variant identification (subtype or recombinant) HIV-1 by phylogenetic analysis.
• Training and consultancy specializing in the field of HIV-AIDS:
....- Development of Training Plans (theoretical and practical) in the HIV field appropriate to the needs and profile of clients nationally and internationally.
....- Consulting services in the development of research projects and scientific papers in the field ....of genetic variability of HIV and antiretroviral resistance to HIV.
....- Consulting services in the design and implementation of molecular biology laboratories and ....viral cultures.


Datos de Contacto
Responsable: África Holguín
Contacto (Telf. /Mail):