Central support unit for translational genomics

Description

This central support unit of Institute Ramón y Cajal for Health Research (IRYCIS) provides integral solutions in Next-Generation Sequencing, including project management and data analysis.
Our high-experienced team perform the analysis of unusual sample types such as microbiota, aptamers or mitochondrial DNA, and handle library construction from different starting material. In addition, the IRYCIS bioinformatics unit offers tailored data analysis solutions (primary, secondary and tertiary) making our genomics platform perfect for complex projects.

Services

Automated nucleic acid extraction
- DNA extraction (blood 350 ul – 5 ml, saliva 2 ml, fresh blood on paper 3,2 mm, fresh tissue 10 mg, FFPE tissue 10 um section)
- DNA amniotic fluid, plasma, buccal swabs, etc.
- Plasmid DNA extraction (1,5 ml bacteria culture)
- RNA extraction (blood 2,5 ml in PAX-gene tubes)
- DNA / RNA viral

Nucleic acids analysis
(Agilent TapeStation 2200 Bioanalyzer)
- DNA analysis by capillary electrophoresis (High-sensitivity or broad range)
- Genomic DNA analysis by capillary electrophoresis
- RNA analysis by capillary electrophoresis (High-sensitivity or broad range)

Nucleic acids quantification
(Qubit 2.0 and Nanodrop)
- DNA quantification by fluorimetry (Qubit 2.0) or UV absorption (Nanodrop)
- RNA quantification by fluorimetry (Qubit 2.0) or UV absorption (Nanodrop)

Massive sequencing
Bookstores Generation
- Human genes panels Re-sequencing
- (PCRs user Multiplex PCRs, Capture)
- Mitochondrial Genome
- Genome microorganisms novo sequencing
- Metagenome 16S
- Virus influenza A
- Aptamers
- miRNAs

Ion Torrent™ Next-Generation Sequencing Technology
- Kit 200 pb / Chip 314 (20 Mb)
- Kit 200 pb / Chip 316 (200 Mb)
- Kit 200 pb / Chip 318 (1 Gb)
- Kit 400 pb / Chip 314 (40 Mb)
- Kit 400 pb / Chip 316 (400 Mb)
- Kit 400 pb / Chip 318 (2 Gb)

Bioinformatics
Secondary analyzes and / or tertiary raw data

Advantages and innovations
Platform.