Microarray Unit

Developed by: Instituto de Investigación Sanitaria del Hospital Ramón y Cajal (IRYCIS)

Description

Summary
Microarrays Unit is specialized in the design and validation of DNA and protein microarrays.

Equipment / Know-How of the relevant group
- Robot for microarrays printing (SpotArray 72, PerkinElmer)
- Reading Scanner (ScaArray Express, PerkinElmer)
- Liquid Handling Robot (Janus Varispan, PerkinElmer)
- Semi hybridizer (BioMixer ™ II, CapitalBio)
- Real-time thermocycler (LightCycler 480, Roche)
- Computer Support for microarray analysis
- Three freezers connected to a radio-controlled alarm system

Services
• Preparation of samples
• Extraction of total RNA (Tripure)
• Extraction of total RNA (Columns)
• Extraction of mRNA (Columns)
• Analysis and quantification of RNA (NanoDrop and gel integrity)
• Microarray
• Printing DNA and protein microarrays
• Retrotranscription and labeling of RNA (two-color labeling with Cy3 or Cy5 without amplification)
• Hybridization (DNA or protein)
• Reading microarrays (DNA or proteins)
• Real-time PCR (LightCycler 480 SYBR green)
• Design of probes (for SYBR green)
• Retrotrancripción RNA
• Real-time PCR (for gene and shown in triplicate)

Applications

Contact

 Javier Martínez Botas

 Phone: +34 91 336 8466

eMail: javier.botas@hrc.es