Confocal Microscopy Unit
Developed by: Instituto de Investigación Sanitaria del Hospital Ramón y Cajal (IRYCIS)
Description
Summary
The
Central Support Unit of Confocal Microscopy provides facilities for
all methodologies related to conventional and confocal microscopy.
Equipment /
Know-How of the relevant group
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Nikon inverted microscope ECLIPSE Ti-e: Objectives Plan Fluor 10X (AN 0.3), 20x
Plan Apo VC (AN 0.75), 40x Plan Apo (AN 0.95) and VC Plan Apo 60X OIL (AN 1.4),
motorized, epilfuorescents block filters.
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Module Nikon Confocal C1: 3 solid state lasers (405 nm, 561nm, 638 nm) and
multiline argon ion laser (488 nm), scanning unit, Detector of three
simultaneous channels.
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Digital camera for photography DS-2MV.
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Cell incubation System for in vivo studies with temperature and humidity control,
and gas perfusion system.
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Training online for job acquisition and image analysis.
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Station off-line dedicated to image analysis.
Services:
•
Confocal Microscopy: confocal microscopy images from fixed samples (Images for multiple
labeling, multi-location (X, Y, Z), FRAP)
•
in vivo Confocal Microscopy: study of in vivo samples (cell culture with
fluorescent labels) for a time sequence through a cell incubation system.
• Conventional and Epifluorescence
Microscopy: Acquisition of images from transmitted light (classical staining,
immunohistochemistry) or immunofluorescence. Big size Imaging
("Stitching").
• Image processing and
analysis.