Developed by: Instituto de Investigación Sanitaria Hospital La Paz
Biomedical research requires the use of techniques and equipment that would be too costly for individual research groups. IdiPAZ promotes the organisation of services or joint platforms that can be used by all interested researchers.This provides technological support to different researchers thereby promoting quality research, improving the profitability of investments in infrastructure and equipment performance, and helping to improve the efficient use of resources.
Flow Cytometry Laboratory
Flow Cytometry has undergone significant advances within the research activity of IdiPAZ, and currently almost all groups involved in research at the Institute use these techniques. The equipment allows for phenotypic analysis of subpopulations, quantification of soluble factors in biological samples and culture supernatants, cell cycle experiments.
Cell Culture Laboratory
Cell culture systems are essential tools for understanding molecular and cellular phenomena which may account for a variety of human disorders. The IdiPAZ Cell Culture Division provides tissue- and cell- related technologies to specialized research groups to explore molecular, cellular, pharmacological, and genetic processes.
In an effort to provide convenient basis to develop Biomedical and Health Sciences Research initiatives, and as part of IdiPAZ ongoing commitment to the scientific community, the Cell Culture Unit was established as a platform to offer technology and professional technical assistance. This principle of action is guided operationally by the access to knowledge, resources, equipment, training and qualified support, that meet the needs of both clinic and basic groups and encourage collaborative alliances. It contributes not only to achieve the goals of translational research but also to the reinforcement of the scientific knowledge, integrating and implementing novel applications and technologies, besides providing guidance to innovation. The activity of this Unit is further supported by complementary actions developed by other divisions and laboratories belonging to IdiPAZ. These provide an interactive and dynamic scientific stage to meet the aims of IdiPAZ and external researchers.
The conception of this Platform, mainly based in a shared organizational structure with a noted interaction with multidisciplinary researchers and other IdiPAZ divisions, guarantees its ongoing growth, development and constant improvement.
The Image Core Unit is located within the IdiPAZ research support platforms to provide researchers with high-end microscopy and histology systems to support bioimaging research. This laboratory offers services for IdiPAZ staff and students as well as other universities, hospitals and external clients. Since Bioimaging techniques are a valuable tool in biomedical and health research IdiPAZ offers the Image Core Unit as a centralized service that provides individual, customized support including project planning, sample preparation, image acquisition and analyses for a wide range of specimens.
The Image Core Unit has two service streams; a full end-to-end service provided by specialist staff, or a “Do it Yourself” service providing access to laboratory facilities, specialist equipment and consumables. In addition, we provide expert service, advice and training for all bioimaging techniques and paraffin. This includes frozen section and specialist histological processing, cutting and staining requirements to obtain the high quality throughput that users need. The Image Core Unit operates on a fee for services basis for all services and laboratory access.
Two laboratories operate within the Image Platform – the Immunohistochemistry and the Image Analyse Laboratories. They both have been committed to excellence, quality, consultation, scientific and technique evaluation and collaboration to produce high quality images with accurate results in an efficient, timely manner. In addition to a wide range of histological services available to researchers, we provide comprehensive immunohistochemistry staining, troubleshooting services and methods for the preparation and staining of cells (cultures, cytospins). Facilities are available for conventional, fluorescence and confocal microscopy, heat induced epitope retrieval, as well as advice and protocols on any area of histology.
The radioactive facility of IdiPAZ laboratories is authorised by the Nuclear Safety Council (Consejo de Seguridad Nuclear, CSN) to operate with non-encapsulated sources radioisotopes: 3H, 33P, 32P, 125I and 51Cr.
Personnel of the Radiological Protection Department of HULP conduct radiological protection and periodically monitor laboratory contamination levels. They are also responsible for the removal and management of waste products and the management of users’ personal dosimeters.
Contains exclusive areas for the use of ß and γ isotopes; aliquot withdrawal and storage of radiolabeled compounds; and equipment for exclusive use with radioactive material.
The laboratory has the following equipment:
• CO2 incubator
• Cell harvester
• Beta counter
• Hybridisation oven
• Gel dryer
• SpeedVac® concentrator
• Screens and containers specifically for γ and β radiation emitters
• High-energy γ and β contamination detectors (Geiger counter)
Automatic Sequencing Laboratory
The goal of the Sequencing Lab is supporting the research by increasing the scientific production. The proximity with the clinical diagnosis strongly implies the Lab in the genetic analysis techniques needed for the diagnosis of genetic diseases, developing in this way an example of translational research.
With the aim of achieving its objectives and supporting the research teams, the DNA Sequencing Lab has technicians and advance equipments for sequencing (Sanger method), genotyping of DNA markers and different molecular biology techniques.
Annual tests of quality control and results homologation guarantee the quality of sequencing and genotyping. It follows internal, national and international (EMQN) tests.
The available equipments are calibrated to detect more than 30 polimorphisms in a single sample, using a maximum of 5 fluorescences at the same time, and making possible the detection of different allelic sizes in the same sample, because of the electrophoretic migration.