Biomarker‐based determination of response to therapy in lung and ovarian cancer
Developed by: Instituto de Investigación Sanitaria Hospital La Paz
Method for determining the response to an antitumoral compound based on platinum, specifically cisplatin, in a patient suffering from lung or ovarian cancer, comprising:
(i) determining the methylation level in the cytosine‐phosphate‐guanine (CpG) island of a gene codifying for a specific microRNA (miRNA) or the expression level of that miRNA in a sample of that patient, and(ii) comparing the methylation level in that CpG island of the gene codifying for that particular miRNA or the expression level of that miRNA with the corresponding reference value.
An increase in the methylation level obtained in (i) or a reduction in the expression level obtained in (ii) compared to the corresponding reference value, enables the early detection ‐in the case of lung cancer‐ and indicates that the patient is resistant to that platinum compound ‐in the case of ovarian cancer.
The miRNA methylation assessment in 36 samples of human lung cancer patients indicates that methylation of this miRNA may be a frequent event in lung cancer patients (53% of the affected individuals); and could have prognostic value in terms of future development of lung cancer as it also appears in samples from patients with non‐malignant diseases (emphysema).
The miRNA methylation assessment in 83 samples of human ovarian cancer indicates that this miRNA methylation is present in 29% of affected women, but however is related to worse response to platinum derivatives‐treatment and less time to tumour progression. Data indicate that 50% of the 30 patients with methylated miRNA receiving platinum‐based therapy relapse before 18 months. Moreover, 75.5% of women without disease recurrence, has unmethylated miRNA. Furthermore, overall survival in women with absence of methylation in this biomarker is 40 months longer than the group of patients with methylated miRNA (> 3 years).Competitive advantages
At present there is no method to select those patients with ovarian cancer who will develop resistance to standard platinum treatment and that are most likely to relapse nor is there the possibility of early detection of lung cancer in individuals with emphysema.This new method allows the early detection of NSCLC and the identification of ovarian cancer patients with worse response rate to platinum or its derivatives.In the case of ovarian cancer patients, this would imply closer monitoring of a selected group of patients with increased probability of tumour recurrence, including its early detection and the selection with more specific treatment regimens.
Estado de protección
Innovation Support Unit
Foundation for Biomedical Research of La Paz University Hospital - FIBHULP-IdiPAZ
Edificio IdiPAZ, Planta Baja
Paseo de la Castellana, 261
Tfno: 91 207 12 34
- Health technology:
- Biotecnología y Diagnóstico Molecular (proteómica, genómica, etc...)