Unidad de microscopía

Desarrollado por: Instituto de Investigación Sanitaria La Fe

Descripción

The Microscopy Unit is a facility that provides to other research institutions and companies,  the necessary infrastructure and experience required for the study, using different microscopic techniques, of biological material from both, patients (biopsies, autopsies ...) and experimental studies (animal, cell culture ...). 

The Microscopy Unit has two different areas of action:

  • Electron microscopy (TEM) area equipped with sample preparation laboratory, ultramicrotomy area, and transmission electron microscope, besides cryomethods laboratory for cryoembedding, polymerization and cryosectioning samples
  • Confocal microscopy area equipped with a confocal microscope Leica TCS-SP5 with incubation system that allows studies in live cells.

EQUIPMENT

  • Transmission Electron Microscope, Philips CM100, equipped with high resolution digital camera (Soft Image System) and the image capture software (ITEM).
  • Ultramicrotome Leica EM UC61I that provides easy preparation of semi- and ultrathin sections, with accesory   cryochamber for cryosectioning.
  • Leica Automatic Freeze Substitution (AFS) for cryo embedding of samples and cryo UV polymerization. 
  • Pyramitome (Leica EM TRIM).
  • Glass Knifemaker for ultramicrotomy (Leica EM KMR2).
  • Confocal Microscope Leica TCS-SP5-AOBS: spectral confocal microscope with resonant scanner mounted on an inverted DMI 6000 CS microscope equipped with 4 laser lines (Ar, HeNe 543, HeNe 594, HeNe 633) and incubation system for short-time live cells studies.

Contact

Responsible Technician:  Mª Pilar Marín PhD

Dirección postal:

e-mail: microscopia@iislafe.es

Telf.: (+34) 618 120 400 (ext. 485642)

http://www.iislafe.es/en/microscopy-unit